pchac mt mkeima (Addgene inc)

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Addgene inc pchac mt mkeima
Pchac Mt Mkeima, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 61 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pchac+mt+mkeima/pCHAC-mt-mKeima+(Plasmid+%2372342)/us12569576-273-14-15
Average 94 stars, based on 61 article reviews

pchac mt mkeima - by Bioz Stars, 2026-09

94/100 stars

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Amplification:

Article Title: PGAM5 aggravated doxorubicin-induced cardiotoxicity by disturbing mitochondrial dynamics and exacerbating cardiomyocytes apoptosis.
Article Snippet: Doxorubicin (DOX), a potent chemotherapeutic agent, is widely used for treating malignancies but is limited by its cardiotoxic side effects.. Mitochondrial dynamics, encompassing fission and fusion processes, play a pivotal role in maintaining cardiomyocyte function under stress, yet their disruption contributes to DOX-induced cardiotoxicity (DIC).. While mitochondrial quality control (MQC) mechanisms are implicated in DIC, the specific molecular players remain unclear.

Article Title: The Integrated Stress Response Suppresses PINK1-dependent Mitophagy by Preserving Mitochondrial Import Efficiency
Article Snippet: .. For constructing mtKeima reporter (pMY004), mtKeima was PCR amplified from pCHAC-mt-mKeima (Addgene, plasmid # 72342) [ ] and cloned into a lentiviral vector pdbr3_CAG. .. For PRKN overexpression (pXG646), PRKN was PCR amplified from CFP-Parkin (Addgene, plasmid # 47560) [ ] and cloned into a lentiviral vector with hPGK promoter and miRFP .

Article Title: Compositions and methods for measuring oxidative stress
Article Snippet: .. A modified mt-Keima cDNA was generated from the mt-Keima open reading frame encoded within pCHAC-mt-mKeima (Addgene, Waterman Mass.) by PCR amplification with primers that include an extra N-terminal MT-COX3 leader peptide to further enhance localization of Keima protein to mitochondria. .. The resulting mt-Keima cDNA PCR product was then subcloned into multiclonal site (5′Xba1, 3′ EcoR1) of the lentiviral vector backbone Ex-EGFP-LV105 (Genecopia, Rockville, MD), which results in the deactivation of EGFP expression to create Ex-mt-Keima.

Clone Assay:

Polymerase Chain Reaction:

Plasmid Preparation:

Article Title: Autophagy promotes cell survival by maintaining NAD levels
Article Snippet: pMXs-IP-eGFP-mAtg5 , Gift from Noboru Mizushima (University of Tokyo) , Addgene Plasmid, #38196. .. pCHAC-mt-mKeima , Lazarou et al. , Addgene Plasmid, #72342. ..

Modification:

Generated:

Transfection:

Article Title: Activation of pro-survival autophagy by a small molecule promoting p62 oligomerisation
Article Snippet: .. The next day, cells were transfected with plasmids containing the packaging gag/pol (retrovirus) or psPAX2 (lentivirus) and envelope pCMV-VSV-G genes, and the YFP-Parkin-IRES-zeo (gift from Douglas Green and Stephen Tait, Addgene, 61728) [ ], pCHAC-mt-mKeima (gift from Richard Youle, Addgene, 72342) [ ], pMRX-IP-HaloTag7-LC3 and pMRX-IB-HaloTag7-mGFP-KDEL (gift from Noboru Mizushima, Addgene, 184901) [ ] or Halo-p62 constructs (ordered from Vector Builders) using Lipofectamine 3000. ..

Construct:

Stable Transfection:

Article Title: Legionella effector Lp PIP recruits protein phosphatase 1 to the mitochondria to induce dephosphorylation of outer membrane proteins
Article Snippet: Cells that survived the hygromycin selection were pooled and screened for expression of the protein of interest after 24 h induction with 1 μg/mL tetracycline (Sigma-Aldrich #T7660). .. For generation of stable cell lines constitutively expressing mt-Keima, retrovirus was packaged in HEK293T cells following co-transfection with 9 μg pCHAC-mt-mKeima (Addgene #72342), 6 μg pUMVC3, and 3 μg pCMV-VSVG in a 10 cm tissue culture dish. .. After 48 h, the viral supernatant was collected, filtered through a 0.45 μm Millex-HV syringe filter unit (Millipore), and used to transduce Flp-In T-REx 293, FLAG Lpg1625 Flp-In T-REx 293, and FLAG Lpg1625 K17A/V19A Flp-In T-REx 293 cells in a 6-well plate for 24 h in the presence of 8 μg/mL polybrene (Sigma-Aldrich #107689). mt-Keima-positive cells were subsequently acquired via fluorescence-activated cell sorting.

Expressing:

Article Title: Evidence that mitochondria in macrophages are destroyed by microautophagy
Article Snippet: .. Plasmids expressing the doxycycline inducible E228Q-VPS4A-HA, mCherry-Tomm20-N10, and pCHAC-mt-mKeima were obtained from Addgene (#200087, #55146, #72342). pLKO.1-puromycin plasmids expressing shRNA targeting to TSG101, CHMP3, VPS4B, VPS4A, NIPNASP1, NIPNASP2, Plscr3 and p62 were purchased from Sigma-Aldrich (TRC number: TRCN0000-054606, −098066, −101824, −101416, −174717, −192544, −105105, −098619). ..

Article Title: Evidence that mitochondria in macrophages are destroyed by microautophagy.
Article Snippet: .. Plasmids expressing the doxycycline inducible E228Q-VPS4A-HA, mCherryTomm20-N10, and pCHAC-mt-mKeima were obtained from Addgene (#200087, #55146, #72342). pLKO.1-puromycin plasmids expressing shRNA targeting to TSG101, CHMP3, VPS4B, VPS4A, NIPNASP1, NIPNASP2, Plscr3 and p62 were purchased from Sigma-Aldrich (TRC number: TRCN0000-054606, −098066, −101824, −101416, −174717, −192544, −105105, −098619). ..

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